Fig. 2

An example of the obtained samples. A A normal sample with hematoxylin-eosin (H&E) staining showing Ganglion Cells(arrow). B An H&E-stained sample of Hirschsprung Disease (HD) demonstrating hypertrophic nerve fibers. C An ICC CD117 IHC sample showing the irregular distribution of ICCs in a HD smaple. D A normal sample of Calretinin IHC demonstrating weak staining of Ganglion Cells