Mismatch repair deficiency in bilateral breast cancer

Table 1 IHC details

Antibody	Clone	Source	Concentration	Antigen retrieval	Interpretation/cut-offs
Anti-MLH1	M1 Roche	Rabbit Monoclonal	1 μg/mL	Cell conditioning 1 (CC1) 92 min, 100 °C	Nuclear expression (Presence/ absence)
Anti-PMS2	A16-4 Roche			Cell conditioning 1 (CC1) 92 min, 100 °C
Anti-MSH2	G219-1129 Roche			CC1 92 min
Anti-MSH6	SP93 Roche			CC1 92 min
Anti-ER	SP1 Roche			CC1	Nuclear expression (> 1% nuclear staining)
Anti-PR	Clone 1E2 Roche			CC1	Nuclear expression (> 1% nuclear staining)
Anti-HER2	Clone 4B5 Roche		6 μg/mL	ULTRA CC1 36 min, 95 °C	Membrane expression (0; 1 + ; 2 + ; -3 +)
Anti-Ki67	Clone 30–9 Roche		2 μg/mL	CC1, 64 min	Nuclear expression (0–20%; > 20%)

ISSN: 2520-8454